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"Protein engineering" analysis of the inserted region shows that it is folded in the transition state.
Nevertheless, the analysis of the inserted amino acid sequence does not display similarity with any known domain.
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Further analysis of these inserted and deleted fragments in these proteins revealed that many of them contained conserved sites, including functionally active sites (Supplementary Table 5 [see additional file 1]).
A BLASTX analysis [ 20] of the inserted sequences allowed us to identify a single LAGLIDADG ORF in each insertion.
Based on the PCR verification, seven transformants were selected for further analysis for expression of the inserted genes.
Analysis of the insert sites in the P. berghei genome showed that insertion occurred exclusively in the expected TTAA insertion site.
A comprehensive analysis of the inserts confirmed by tests on laboratory stands, showed the interaction between the thermal fatigue and abrasive wear, combined with the process of oxidation.
Analysis of the insert DNA sequence as described above, revealed an ORF (1890 bp) with ATG as start codon and TAG as termination codon.
Colonies were picked and plasmids isolated by standard miniprep following restriction analysis of the insert.
Further analysis of the insert sequences identified through the different genetic selections did not reveal the presence of common "seed" regions or extended sequence similarity or complementarity with previously reported human miRNAs.
Analysis of the insert size distribution showed that the overlapping ditags tended to be small fragments.
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