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Cross-hybridizing probesets (represented by the _s_, _x_ and _a_ identifiers) were removed and BLASTn analysis of the express sequence tags (ESTs) on which the remaining probesets were designed was conducted against the current NCBI RefSeq V. vinifera mRNA dataset using PERL.
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Fig. 1 SDS-PAGE and zymogram analysis of the expressed xylanases.
Proteomics offers scientists unprecedented power for the analysis of the expressed genome.
Frequency analysis of the expressed fitness for use (FfU) responses was performed on the individual response data, i.e. using all of the individual responses corresponding to the individual documents, per workshop scenario (Fig. 4).
The correctness of the constructs was confirmed by sequencing and Western blot analysis of the expressed proteins.
Immunoblot analysis of the expressed proteins showed that the absence of fluorescence for YN-eIF4ER/YC-VPg could not be attributed to reduced protein accumulation (Figure 2 D).
Sequence analysis of the expressed endogenous TCRs strongly suggested that RAG-dependent TCR recombination occured in the RAG-knocked out (KO) strains used.
Analysis of the expressed proteins by fluorescence microscopy revealed that while GFP and GFP-IC distributed diffusely throughout the whole cell when individually expressed, muNS and muNS-Mi accumulated into large cytoplasmic inclusions (Figure 3B).
Importantly, analysis of the Expressed Sequence Tag (EST) database indicated that the SUV420H1_v2 mRNA is generated in vivo and was detected in multiple tissues from diverse species (data for mouse and human is shown in Figure S1C).
cDNA sequence analysis of the expressed transcript confirmed the identification as MYB110a.
Functional analysis of the expressed genes was conducted based on "TIGRFAMs" http://www.tigr.org/TIGRFAMs/index.shtml.shtml
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