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To date, there has not been a systematic analysis of the downstream substrates of such receptor-activated SFKs.
In fact, unsupervised clustering identifies four clusters and broader phenotypic and genetic differences distinguishing these four transcriptomal groups have been reported.[26] Analysis of the downstream signaling components of the PDGF proteomic group revealed generally lower PI3K/Akt activity than in the EGFR glioma group although S6 phosphorylation was paradoxically high.
Further analysis of the downstream cell cycle modulators known to be influenced by Akt signalling was also undertaken.
These three data sources will provide sufficient data to inform a detailed analysis of the downstream cost consequences for the different treatment groups.
Thus, analysis of the downstream genes without discriminating between transcription from their own promoters or by read-through from upstream genes might result in a false categorization.
The inhibition of hPRL-R signaling by these three sABs was confirmed by western blot analysis of the downstream components of the prolactin induced signaling pathway.
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Using the knowledge database of Ingenuity Pathway Analysis, one of the downstream components affected at the expression level, which was also on both the MDA (Additional File 4) and MCD (Additional File 7) lists, was COL1A1.
The analysis of the Akt downstream signaling pathways suggested that Akt2 specifically targets and activates the p70S6K signaling pathway.
Analysis of the 59 downstream mRNAs confirmed an overall ER stress activation upon LG growth condition.
This is supported by analysis of the genes downstream of the NUP62 orthologous genes in non-eutherian species.
PCR analysis of the genes downstream of the NHS gene, SCML1, RAI2, CXorf20 and SCML2, revealed an extensive segmental deletion of ∼0.9 Mb.
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