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A minimum of five consecutive breaths were selected for analysis of the digitised VT signals.
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This was confirmed by image analysis of digitised images to measure the nuclear to cytoplasmic fluorescence ratio (Fn/c, Methods: Figure 1C; where Fn/c > 1 indicates nuclear accumulation), indicating a significantly higher level (c. 2 fold, p < 0.01) of nuclear accumulation in the tumorigenic SAOS-2 cells, compared to the non-transformed SR5 cells.
For automated image analysis of digitised slides were accessed through the Slidepath Image Analysis system (Leica Microsystems GmbH, Wetzlar, Germany) and evaluated with the program's nuclear (for Ki-67), cytoplasmic (for CD68+) and membranous (for CD8+) scoring algorithm.
Computer analysis of digitised mammograms is now remarkably sensitive for the detection of calcifications and masses.
Semi-automated analysis of digitised analogue mammograms has previously suggested that local increases in density may occur prior to cancers being detected.
Fasanella et al. used computer-assisted image analysis of digitised slides, and found manual and automated methods to be comparable in assessing Ki67 expression in breast cancer [ 8, 30].
In addition, much of the digitised rainfall data from numerous stations are viewed as being unreliable, with important events missing and errors in the digitised data.
This will be followed by a brief description of the digitised network that was used.
These amendments were incorporated into the final version of the digitised questionnaire.
Some older publications that are not in copyright are being digitised, but serious issues remain over the rate at which the historic legacy literature is being captured, the completeness of the digitised literature and ease of access to this literature.
The availability of software to automatically measure P0.1 using digitised signals from standard ventilators [ 10] has contributed to the importance of P0.1 in the analysis of the decisional algorithm of mechanically ventilated patients.
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