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Therefore in addition to the MLST analysis of the CF strains, we determined the MLST profiles of 72 colonizing S. agalactiae isolates, which were collected recently, as a reference population [8].
An extensive molecular analysis of the CF transmembrane regulator (CFTR) gene was performed to establish the CFTR mutation spectrum and frequencies in the Palestinian population, which can be considered as an understudied population.
In general the Reg/PAP genes are coordinately upregulated by stress to the pancreas [ 1] but this does not always hold as shown by microarray analysis of the CF mouse pancreas.
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The influence of other relevant parameters from the FFs, such as amount of water consumed, hydraulic conductivity, water depth, and SW flow volumes, were integrated into the sensitivity analysis of the CFs.
In order to explore the extent of clonality and the epidemiology of the P. aeruginosa collection, we carried out a PFGE analysis of the 38 CF isolates.
Biological replicates were generated by combining analysis of the three CF patients' samples.
Normal tissue evaluation was carried out using 2D-ROI analysis of the lung, muscle (cf Koh et al, 1992) and a body area 15 mm above the heart (cf Bentzen et al, 2000), and normal tissue to heart mean activity ratios were calculated.
A recent analysis of the genomes of various CF and non-CF P. aeruginosa isolates revealed mosaic structures, consisting of a conserved core component interrupted by strain-specific genomic islands acquired by HGT, which seem to provide CF isolates with specific metabolic pathways involved in infection [74].
One study recovered 6 isolates from the analysis of 258 CF respiratory tract specimens [5].
Sixteen unrelated homozygotes for c.1521_1523delCTT in CFTR with known basic defect and their parents were included into the analysis of the manifestation of the CF basic defect.
The bounding boxes obtained by segmentation can then be used for an independent analysis of these elements (cf. Section 3). 2. Reference-based assessment of quality Measurement of color rendering accuracy by analysis of the extracted reference targets (cf. Section 4.1).
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