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DOI: http://dx.doi.org/10.7554/eLife.01584.008 10.7554/eLiFigure84.009 Figure 2 figure supplement 1. Hydrogen-deuterium exchange analysis of Spy and the Spy-Im7 7-45 complex by electron capture dissociation fourier transform ion cyclotron resonance mass spectrometry (ECD-FTICR-MS).
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Between 1932 and 1936, the Cipher Bureau took on additional responsibilities, including radio communications between military-intelligence posts in Poland and abroad, as well as radio counterintelligence — mobile direction-finding and intercept stations for the locating and traffic-analysis of spy and fifth-column transmitters operating in Poland.
A "comprehensive analysis" of how human spying operations were being mismanaged was spelled out in a classified annex to the report that cannot be made public, the committee said.
But the Russians refused to provide details, Tenet wrote, and "in the final analysis, it was still a game of spy versus spy".
Analysis of the reasons behind the improved chaperone ability of activity enhancing mutants of Spy should inform us about Spy's catalytic mechanism and perhaps also tell us what makes for a good chaperone.
The flexibility of Spy variants is interpreted as the percentage of protected protons in the deuterium exchange analysis measured at 25°C and is inversely correlated with the stability of these Spy variants in the absence of client.
Sequences analysis of the PCR products corresponding to filled and empty Spy sites further confirmed that the integration of Spy occurs precisely between 5′-AAA-3′ and 5′-TTT-3′ host nucleotides, without deletion or duplication of target sequence (fig. 2 B).
It will be a kind of Spy vs. Spy.
U.S. author of spy thrillers.
Here we review the origin and mechanism of SpyTag/SpyCatcher, focusing on recent innovative applications.
(A ) Surface properties of Spy.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com