Your English writing platform
Discover LudwigExact(25)
c, RT qPCR analysis of reporter mRNAs.
m, RT qPCR analysis of reporter mRNAs.
e, RT qPCR analysis of reporter mRNAs.
Any deliberate intervention into the regulatory elements requires a subsequent evaluation based on analysis of reporter proteins.
These results were further corroborated by quantitation of vector genome copies and histological analysis of reporter (tdTomato) gene expression.
For analysis of reporter mRNA levels (Figs. 1B, 2, 3), HeLa cells were transfected in 12-well plates by scaling up the plasmid transfection protocol detailed above.
Similar(35)
Here, using unbiased forward genetic screens, followed by analysis of reporters and engineered mutants, we show that stomatal initiation in the grass Brachypodium distachyon uses orthologs of stomatal regulators known from Arabidopsis but that the function and behavior of individual genes, the relationships among genes, and the regulation of their protein products have diverged.
Furthermore, in our comparative analysis of reporters, p21PΔp53-luc p21PΔp53-lucbinding sequence, as with p21P-lackingSmad downstream 4*SBE-luc reporter, show significant inductions by zinc, but not the p53-binding reporter thas is only activated by p53, providing evidence that zinc treatment induces p21 with/Cip1 transactivation in a Smad-dependent and p53-independent manner.
Transient transfection of such reporter vectors into cultured cells and quantitative analysis of the reporter gene product is a fast and efficient way to study disease gene expression.
Our results (figure 2) were highly concordant with the transcriptional pattern of YUC1/ YUC4 genes (the Arabidopsis homologous to tomato ToFZY gene) reported by Cheng et al. [ 33] based on histochemical analysis of GUS reporter lines and in situ hybridization.
Wang, H. & Jiang, L. W. Transient expression and analysis of fluorescent reporter proteins in plant pollen tubes.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com