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This corresponds to results from repeated PB flow cytometry analysis of recipients within the 7-month period where no eGFP-positive or CD45.2 donor cells were found (not shown).
While designed to predict risk of non-relapse mortality (NRM), a recent retrospective analysis of recipients of myeloablative allogeneic stem cell transplant (SCT) demonstrated that a higher HCT-CI was predictive of 30-day readmission, and that 30-day readmission was, in turn, an independent predictor of all-cause mortality.
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Figure 7 shows analysis of recipient mice that received populations of CD4+Ly5.1+/−Foxp3GFP- and CD4+ Ly5.1+/+Foxp3GFPhi cells.
After 4 hours peptide specific lysis of the transferred cells was determined by FACS analysis of recipient splenocytes.
Immunohistological analysis of recipient mice receiving only PBS revealed only scattered human T cells but these cells were readily identified by FACS analysis of collagenase digested spleens.
Within one to two hours after transplantation, microscopic analysis of recipient testes demonstrated that donor spermatogonia were present in the lumen of seminiferous tubules (Fig. 1h).
The analysis of recipient testes by fluorescence microscopy at 1 and 14 h post-transplantation revealed the presence of donor germ cells in the lumen (Fig. 2a b) and in contact with recipient Sertoli cells (Fig. 2c d).
Analysis of recipient mice 16 weeks after transplantation showed that CD41+ cells, c-Kit+ cells, CD34+ cells, and CD45− cells were enriched in long-term repopulating activity (Fig. 4B).
(4) The analysis of recipient population's perception of health services provided allow to better tailor and adapt the health services offer to the population's needs and expectations.
Complete blood count analysis of recipient peripheral blood 3 months after transplantation showed that various blood cell lineages were successfully reconstituted by both the WT and Smurf2-deficient donors (Fig. S5C).
In contrast, cumulative analysis of recipient plasma from both laboratories identified pregnancy predictive profiles with an AUC higher than those from each laboratory analyzed apart, suggesting that FTIR can be an interesting, simple tool to select recipients on field in conventional MOET programs.
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