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Analysis of promoters recognized by PvdS, an extracytoplasmic-function sigma factor protein from Pseudomonas aeruginosa.
In this review, we present a comprehensive analysis of promoters and their underlying mechanisms in transcriptional regulation, including epigenetic marks and chromatin-based regulation.
The genome-wide identification of transcription start sites, enabled by high-throughput sequencing of a cDNA library enriched for native 5′ transcript ends, is ideally suited for the analysis of promoters.
Analysis of promoters of RPs led Schaap and van Poppel to postulate that two conserved rp gene promoter cis elements, TRP1 and TRP2, regulate expression of rp genes, and thereby, biogenesis of ribosomes [49].
Simply stated, since the traditional experimental analysis of promoters typically covers regions of about 1000 2000 bp, there is no discovery bias that prefers the first 200 nucleotides upstream to the TSS over say the next window of 200 400 bp, or even 400 600, whereas our main results indicate a very strong bias to the first 200 bp, not the first 2000.
TF-binding site analysis of promoters for genes that were up-regulated within the FPSC (the UGE-only cluster) identifies two transcriptional regulators of the cell cycle, namely E2f and Nfy (Tables 2, S13), consistent with over-representation of self-renewal genes (Tables 3, S7) [20].
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e, Paired analysis of promoter:intron CDTS percentile.
Interestingly, the analysis of promoter regions for NAC, WRKY and β-glucanase genes revealed the presence of stress-responsive cis-elements, such as GCC-box (data not shown), known as recognition sites for ethylene-responsive transcription factors41.
Analysis of promoter-specific CIITA isoforms revealed that types I, III and IV all were decreased by HCMV, a result that differs from changes after incubation of these cells with lipopolysaccharide (LPS).
Elucidation of regulatory mechanisms at the gene expression level via analysis of promoter regions is a prominent procedure to decipher such gene regulatory networks.
For the analysis of promoter binding the promoter microarray design for X. tropicalis can be used.
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