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This possibility is also due to technological advancements: WGA of single cells provides large DNA quantities from practically any desired cell [28], and high-throughput DNA analysis technologies enable cheaper and quicker analysis of multiple loci obtained from WGA.
Because analysis of multiple loci from single cells requires a minimal amount of DNA (typically ∼1 µg in our case) we first amplified genomic DNA either by ex vivo culturing of single cells or by WGA (as described above), or a combination of these.
The EBSP builds on the Bayesian Skyline Plot (BSP) [ 14] in several ways: Permits analysis of multiple loci.
This study adds to a growing list of studies that support predictions of Haldane's Rule using cline analysis of multiple loci of differing inheritance modes.
This paper extends the BSP further by allowing the joint analysis of multiple loci and eliminating the requirement to pre-specify the model dimensionality.
This study adds to a growing list of studies that support predictions of Haldane's Rule using cline analysis of multiple loci of differing inheritance modes, although alternate hypotheses like selection on different mtDNA types cannot be ruled out.
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KJ carried out the linkage analyses and significance testing, helped with organising and writing statistical parts of the manuscript, and together with AA, shaped the statistical analysis of cosegregation of multiple loci.
This approach, called Bayesian Estimation of Species Trees analysis [ 24], thereby avoids concatenation of multiple loci, and estimates a species tree based on a model that accounts for deep coalescence of gene trees.
The forward primer of each locus was 5- prime end labelled with one of four florescent-labelled dyes (FAM, NED, PET or VIC) to enable multiplexing of multiple loci during fragment analysis.
Haplotype analysis assessed disease susceptibility more powerfully by analyzing the combined action of multiple loci.
The length of the single locus equals the total length of multiple loci (300 bp each).
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