We have also prepared molecular beacon DNA biosensor arrays for simultaneous analysis of multiple DNA sequences in the same solution.
However, the specific analysis of multiple DNA adducts is still in its early stages.
This is in line with the earlier studies suggesting that running the same DNA pool in multiple arrays should be preferred over construction and analysis of multiple DNA pools within the same array [ 18, 22, 32].
Two important features of QMC-PCR are (1) the production of initial PCR products from genomic template of varying availability and (2) the use of a resulting PCR product as template for analysis of multiple DNA regions with nested primers.
SMRT analysis enabled the identification of multiple DNA methylation motifs, some novel, including the confirmation of a cytosine methylation motif which enables the evasion of the endogenous type II endonuclease.
We therefore searched for latent periodicity by identifying significant heterogeneities (at level α = 10−6) in overlapping windows of various sizes, in analysis of multiple-scanned DNA sequences with variable steps.
For example, a recent protocol in our laboratory required from the same sample the analysis of multiple biomarkers (RNA, DNA, and protein) including one predictive biomarker to identify eligible patients.
Recent developments in tissue microarray technology have enabled the analysis of multiple targets at the DNA, RNA or protein level on sections containing hundreds of tumour samples.
APEX array technology facilitates the simultaneous analysis of multiple mutations through hybridization of fragmented template DNA to specifically designed primers, followed by single nucleotide extension at the site of each putative mutation [8], [9].
This possibility is also due to technological advancements: WGA of single cells provides large DNA quantities from practically any desired cell [28], and high-throughput DNA analysis technologies enable cheaper and quicker analysis of multiple loci obtained from WGA.
