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By enabling the culture-independent genomic analysis of microbial populations, metagenomics "offers a window on an enormous and previously unknown world of microorganisms" (Handelsman [2007], 8).
DK-MICROBE may also be useful for analysis of other human diseases and for analysis of microbial populations in various microenvironments.
The cecal contents of animals euthanized at 4.5 months after surgery were collected for analysis of microbial populations by pyrosequencing performed by the Core for Applied Genomics and Ecology (CAGE, University of Nebraska) using a Roche Genome Sequencer GS-FLX.
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Huse, S. M. et al. VAMPS: a website for visualization and analysis of microbial population structures.
Included in its activities is Visual Analysis of Microbial Population Structures (VAMPS) at http://vamps.mbl.edu.edu
Tag sequences are publicly available online through the visualization and analysis of microbial population structures (VAMPS) project of the Marine Biological Laboratory, Woods Hole, Massachusetts (http://vamps.mbl.edu).edu
BIOM: Biological Observation Matrix; QIIME: Quantitative Insights Into Microbial Ecology; MG-RAST: Metagenomic Rapid Annotation using Subsystem Technology VAMPSS: Visualization and Analysis of Microbial Population Structures; OTU: Operational Taxonomic Unit; API: Application Programmer Interface; JSON: JavaScript Object Notation; GPL GNU Public Licensee.
Sequence QC and analyses were performed through the Visualization and Analysis of Microbial Population Structure (VAMPS) Web site (http://vamps.mbl.edu/index.php) and as described by Huse et al.
All amplicon datasets were downloaded from the Visualization and Analysis of Microbial Population Structures database (https://vamps.mbl.edu) and analyzed using the same methods as those applied on the SCS sample (i.e., using SILVA-ngs to generate OTUs and identify taxonomy; using Mothur to estimate diversity indices by resampling with 1000 iterations).
Here, we outline digital image analysis methods to quantify the coaggregation, mutual avoidance, or random distribution of microbial populations in biofilm and flocs.
Therefore, these arrays are confined to diversity analysis of selected microbial guilds, while PhyloChips are best suited for detecting changes in the taxonomic composition of microbial populations.
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