Some limitations to the laboratory methods were reported in the use of type-specific PCR primers, in situ hybridization, or Southern blot analysis and resulted in the biased detectability of individual types [ 7].
Future work should address the question as to whether analysis of individual cell types derived from the synovium might yield better predictive biomarkers.
The analysis of individual cell types from the airways and lungs of humans did indeed reveal that the expression profile of each miRNA differed significantly depending on the cell type.
However, when only the most highly differentially expressed genes are of interest, it may not be necessary to undertake time-consuming and costly separation and analysis of individual cell types.
The challenge for proteomic studies of the tumor microenvironment stems from the cellular complexity of the microenvironment, necessitating separate analysis of individual cell types.
Effective feature selection and mathematical modeling strategies should be developed in the analysis of individual data types and in the integration thereof.
While some studies may require analysis of individual cell types, if all these populations can be studied together, the cost savings is substantial.
Furthermore, although a strong overall inflammatory cell infiltrate is a superior predictor of prognosis than the analysis of individual cell types, high numbers of lymphocytes and plasma cells in particular are beneficial to survival and associated with a number of favourable pathological characteristics.
In the analysis of individual HPV types, there was weak evidence of an association between incident GWs and cervical HPV-52 DNA at enrolment (aHR 3.81, 95% CI: 0.90 to 16.23, P = 0.07), with one lesion occurring in a woman co-infected with HPV-6.
Participants report the number of hours spent in each activity per week, allowing for analysis of each individual type of activity as well as a summation of all four categories of activities to determine the participant's total amount of physical activity over the past year [ 95].
