Sentence examples for analysis of hunk from inspiring English sources

For analysis of Hunk expression in mouse tissues one μl of cDNA was used as a template for PCR amplification with primers 5′-agatccagcagatgatccgac-3′ and 5′-tagcgctcaagtttcttgttcaa-3′ and Platinum AccuPrime DNA polymerase (Invitrogen).

qRT-PCR analysis confirms the transcriptional activation of Hunk in these settings, indicating that Hunk transcription is coincident with Wnt signalling activation and tumour formation.

Affymetrix microarray analysis indicates an acute transcriptional activation of Hunk following the loss of Apc in the intestine and liver and in colonic adenomas from the Apc Min mouse.

To further investigate the role of HUNK in cell survival, we examined MGF isolated from Hunk WT and Hunk KO mice for survival response.

Indeed, analysis of the Oncomine database confirmed the association of Hunk expression and intestinal cancer.

However, the physiological role of Hunk has been largely elusive since its identification.

Equal numbers of Hunk WT and Hunk KO MGF were plated and then assessed by trypan blue exclusion.

Equal numbers of Hunk WT and Hunk KO cells were transfected with GFP-LC3 and treated with chloroquine the following day for 4 h.

Equal numbers of Hunk WT and Hunk KO cells were plated and the following day treated with vehicle (water) or the autophagy inhibitor, chloroquine for 4 h.

Indeed a Tcf/LEF binding motif can be found within the promoter region of Hunk.

Moreover, studies using Hunk− / − (Hunk KO) mice show that normal mammary gland development is altered by loss of HUNK function during postlactational involution, a stage of mammary gland development governed by apoptotic clearance of mammary epithelial cells, where Hunk KO mice display increased levels of apoptosis during involution [ 5].