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The experimental data were used to validate a CFD model for the analysis of bypass flow characteristics in detail.
Analysis of bypass products captures the outcome of both insertion and extensions so some differences from the results of individual insertion and extension experiments are anticipated.
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Analysis of the bypass products revealed that the nucleotide inserted opposite the lesion site was complementary to the nucleotide on the 5′ side of the PdG lesion of the template strand; subsequently, the primers were extended, generating products with either mismatches or deletions.
Additional methods of accelerating the analysis include setting various restrictive filters before the analysis to bypass the calculations that are not of interest to the user.
A preliminary numerical analysis of the proposed bypass geometry was also presented.
The comparison revealed that the scaling parameters of direct ECC bypass are well preserved in the prototype and reduced models, from which we conclude that the modified linear scaling methodology is appropriate for designing a reduced test facility and for a scaling analysis of direct ECC bypass in the reflood phase of an LBLOCA.
Primers sequences, number of sequences analyzed by HT-SOSA, calculaterrorratesates, lesion bypass graphs, and lesion bypass analysis of the combination of hPolη, hPolκ, and hPolι.
However, Dpo4-catalyzed bypass of 1, N-ε-G yields almost entirely −1 frameshift products by LC−MS/MS analysis, whereas bypass of M1dG resulted in a substantial fraction of the products representing accurate bypass.
Analysis of electron bifurcation and bypass reactions in cyt bc1 complexes from yeast mutants that mimic β- and γ-proteobacterial and actinobacterial homologs is ongoing to decipher the underlying structure/function relationships.
Although RFLP methodology has shown great discriminatory capacity and has been the most effective genotyping method for M. tuberculosis and many other pathogens, newer DNA sequence-based genotype methods should allow seamless computerization and objective analysis of results that would bypass many of the limitations described in this study.
For the development of a rapid platform for translational analysis of genetic sequences by bypassing time- and labor-consuming steps of cell-based gene expression methods, we attempted in this study to combine the techniques of gel electrophoresis and cell-free protein synthesis in a streamlined manner.
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