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In mutants lacking the Crumbs1 gene, lesions are commonly sparse (Fig. 5 A), which makes an histological analysis laborious and demanding.
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Whereas, the process of morphometric analysis is laborious as it requires ample amount of time for accurate and precise landmark annotation.
However, this analysis is laborious and costly as well as inaccessible for most clinical laboratories.
Because the manual analysis was laborious and time-consuming and comparison to MG1655 produced many false positive and negative differences, we sought a better approach.
Genotyping by RFLP analysis is laborious, providing a bottleneck for mapping studies.
Conventional chromosome analysis is laborious, however, and requires highly trained personnel.
However, conventional DNA sequence analysis is laborious and expensive for long transgenes, such as lacZ (3096 bp), and is not normally implemented in routine screening.
Although 2D-PAGE analysis is laborious, 2D-PAGE has been applied for observing oxidative damage of ApoA1 [ 67] and glycation [ 68] with high protein separation efficiency.
However, it has been difficult to determine allele loss in cHL cases because CGH analysis is laborious and requires large numbers of micro-dissected cells [ 12].
The traditional detection and quantitation of phosphorylated MAPKs via Western blot analysis is laborious and somewhat arbitrary in the designation of appropriate densitometric backgrounds for comparison, especially in situations where the significant activation response is not large.
Traditionally, capillary gas chromatography combined with mass spectrometry (GC MS) has been used in doping analysis including laborious sample preparation for analytes or metabolites with a polar, non-volatile, or thermolabile nature [ 2].
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