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This slow growth makes it difficult to carry out genome-wide synthetic genetic analysis in this background.
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However, we could not directly examine the role of LIG3, because its inactivation together with BLM and TRF2 led to cell cycle arrest (data not shown), which prevented fusion analysis in this genetic background.
The analysis in genetic background revealed that Hua1228S covered up to 87.6% of the recurrent parent genome (Guangzhan63-4S), while 4.5%and7.9%9% of genomes from the donors VE6219 and YR7029 39, respectively (Fig. 2).
For data analysis in ImageJ, background was estimated from the adjacent negative control lane loaded with repurified GFP-PHOSBP.
Based on the permutation test, the optimal threshold of the Pearson correlation coefficient (PCC) values for co-expression analysis in the japonica background (rice lines Mudanjiang 8 and Rb49) and indica background (rice lines IR24 and IRBB13) were determined as 0.66 and 0.73 with a false discovery rate of 0.001, respectively (Additional file 1: Figures S3 and S4).
Analysis of a clinical population referred for language analysis in terms of background variables and extent of language problems.
The proviral genome was engineered to facilitate expression of diverse HIV-1 env sequences, allowing analysis in an isogenic background.
Since our aQTL analysis treats each locus as independent, we performed the same analysis in the RM background.
As homozygous expression of the Nlk gene trap alleles is lethal, we carried out our analysis in the heterozygous background.
To perform the analysis in an isogenic background, RNA and DNA were isolated from SCC, AK and normal (unexposed) epidermis (NS) from each of 13 OTRs.
However, the extent and specificity of this proposed activity of SbEhd1 will require further analysis in backgrounds where SbCO has minimal influence on the expression of these genes (Sbco-3 backgrounds).
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