As an example, for Cj0685c, only two homopolymeric tract lengths are observed in our analysis (in-frame and one base deletion frameshift), and this bi-allelic distribution is indistinguishable before and after mouse-adaptation.
With complementary DNA analysis, in-frame skipping of exon 12, with the loss of 24 amino acids (p.T381-N404del), was demonstrated for the c.1071A > G (p.A358A) synonymous substitution.
Guan, B., Gao, M., Wu, C.H., Wang, T.L. & Shih Ie, M. Functional analysis of in-frame indel ARID1A mutations reveals new regulatory mechanisms of its tumor suppressor functions.
Consistent with the results from the Tn analysis, an in-frame deletion in pssY did not render cells 'heavy'heavy
UBE3B transcript analysis revealed in-frame exon skipping in the affected animals resulting in an altered protein lacking 40 amino acids, of which 20 are located in the conserved HECT-domain, the catalytic site of the UBE3B protein.
The chapter describes some critical issues associated with the practical use of advanced analysis methods in frame design and provides some simple solutions for its practical implementation.
The problem becomes amenable to a complete analysis in the frame of the diffusive-thermal approximation when a simplified flow field and the flame-sheet combustion model are used.
However, we carried out RNA extraction from 10-day old biofilms and semi-quantitative reverse transcriptase PCR analysis showed that in-frame GFP mRNA was present in the biofilm, but was absent from planktonic cells incubated for the same period in shaking culture (data not shown).
The MET PCR product from E98 was 240 bp smaller than that from U87 and CaCo2 cDNA (Fig. 2a), and Sanger sequencing analysis revealed an in-frame deletion of nt 2050 2289 in the coding sequence, corresponding to exons 7 and 8.
Each resolved clone was subjected to DNA sequence analysis to confirm the in-frame insertion.
