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The researchers have started testing other resins without uranium and formaldehyde issues and plan to include that analysis in a final report slated for late 2013.
Six ng of each cDNA were then used for real-time PCR analysis in a final reaction volume of 20 μl.
Quantification of gene expression was performed using qRT-PCR analysis in a final reaction volume of 10 μl, consisting of 1× SYBR Green Supermix (Bio-Rad, Hercules, USA), 6 μM forward primer, 6 μM reverse primer (Table 2) and 5 ng cDNA.
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Students will develop and test hypotheses, provide scientific evidence and analysis, culminating in a final presentation.
Closures and weakly covered regions were verified by PCR and sequence analysis, resulting in a final error rate of below 1 error per 10,000 bases [as estimated by CONSED [69]].
Multivariate analysis resulted in a final model of five prognostic variables for DFS and four prognostic variables for OS.
Those with at least one follow-up record in NHEFS were included in the analysis, resulting in a final sample size of 6,046.
This sample was an outlier, and consequently this mother and her infant were excluded from the analysis, resulting in a final sample size of 113 mother infant pairs.
This sample size was doubled in order for the age groups to be dichotomized during logistic regression analysis resulting in a final total sample size of n = 258.
Two hundred and ninety-two cases (4.6%) had both pulmonary and extrapulmonary disease and were excluded from analysis, resulting in a final study population of 6,124.
These subjects were excluded from the analysis, resulting in a final sample size of n = 1,882 at wave 3 (wave 4: n = 1,575; wave 5: n = 1,219).
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