Consistent with the results of the primary analysis, having difficulty accessing syringes partially mediated the association between frequent MA injection and syringe sharing (Sobel test P-value = 0.053).
For comparison between Pfam22, Pfam24 and the two HMMER releases (HMMER2.3.2 and HMMER3b3), we removed the top 20, promiscuous conserved domains, as well as Zinc Finger domains for the analysis, as HMMerThread has difficulties of identifying the correct family of Zinc Fingers.
However, it should be noted that this type of analysis has many difficulties.
For example, Pcd mice have been shown to exhibit short and irregular strides recorded by footprint analysis, to have difficulties keeping balance on the rotarod and to display reduced open field locomotion activity (Triarhou et al. 1996; Wang et al. 2006).
The merit of LSM is that one equation is obtained from geometric analysis, but it has difficulty incorporating lens distortion.
The process of this analysis has highlighted the difficulties in obtaining an accurate overview of brain drain.
Furthermore, pyrazines produced by microorganisms can be found in low quantities in culture media (Beck et al. 2003; Dickschat et al. 2010; Nawrath et al. 2010), and the bottleneck of analysis has been the difficulty of sample preparation due to the high solubility and volatility of pyrazines in microbial samples.
Despite recent advances for fluorescently labelled growth cones [ 48], fine analysis of filopodia is still limited by the difficulty in obtaining large datasets: fully automated image analysis techniques have difficulty with phase-contrast imaging at this level of detail, and hand-tracing is prohibitively time-intensive.
Due to the intrinsic limitations of such spatial space analysis, these methods have difficulties handling statement reordering, insertion and control replacement.
Clustering analysis has following common problems: difficulty in handling mass data and distribution data, difficulty in determining parameters, low efficiency and poor clustering quality.
