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For our analysis, grades (+1) and (+2) of Set B were merged into a single grade while retaining the other grades as used by the respective sources.
Within multivariate analysis, grades 3 4 oral mucositis, grades 1 2 nausea/vomiting and grades 1 2 thrombocytopenia were associated with improved survival.
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In brief, after 1 hour incubation in 3.0 ml of 68% trace analysis grade (TAG) HN03, 2.0 ml 30%H2O22 (both Fisher Scientific UK Ltd ,Loughborough, UK), and 3.0 ml milli-Q water (18.2 MΩ cm), acid-digestion was accelerated by microwave-heating (Anton-Paar, 2000) for 45 mins.
All other chemicals were either reagent or analysis grade.
All solvents were Carlo Erba (Milan, Italy) pesticide analysis grade.
Acetic anhydride and anhydrous pyridine were obtained from Merck (Darmstadt, Germany) and was pro analysis grade.
All solvents (trace analysis grade), 0.1% formic acid in water and LC-grade water were purchased from Baker (Quebec, Canada).
Organic solvents (methanol, distilled water) of GC analysis grade were purchased from Burdick & Jackson (Philipsburg, NJ, USA).
Other solvents and reagents (trace analysis grade), methanol (MeOH), ammonium hydroxide (NH4OH) and HPLC-grade water were purchased from Fisher Scientific Inc. (Whitby, ON, Canada).
All derivatization reagents, high-performance liquid chromatography (HPLC -grade water, sodium HPLC -gradeNaOH) and hydrochloric acid (HCl) (37 %), dioxin analysis grade dichloromethane (DCM), and methanol waterobtained from Wako Chemical.
Internal standards used included scandium (Sc, 100 µg/L), rhodium (Rh, 20 µg/L), germanium (Ge, 20 µg/L) and iridium (Ir, 10 µg/L), prepared with 2 % trace analysis grade HNO3 (Fisher Scientific, UK).
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