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Experimental confirmation of key pathways were performed by a preliminary proteome analysis, fermentation tests with and without 2-Deoxy-D-Glucose and/or C flux analysis of CO2 supplemented fermentation.
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The importance of signal noise generated by these physical/chemical processes is discussed based on a time constant analysis of fermentation processes with yeast and filamentous fungi.
A complete system for on-line sampling, sample handling and analysis of fermentation processes has been set up.
The preliminary analysis to fermentation performances showed that ORP and H2/CO2 ratio were both at low levels when using cassava (Figure 2).
AS and NL carried out the analysis of fermentation products.
Analysis of fermentation samples has been described previously [ 22].
Samples for analysis during fermentation were collected via the sterile sampler on the fermentor.
Isolates were identified as F. tularensis subsp. tularensis (type A) by biochemical analysis (glycerol fermentation).
Growth of bacteria was monitored by analysis of fermentation products and determination of optical density of the cultures (OD600).
JGE and AMG designed and carried out the analysis of fermentation products and participated in writing the manuscript.
Here again, such metabolite distribution resulted from the delay between metabolite analysis and fermentation achievement (4 days).
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