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Fig. 4 Real-time PCR analysis expression of the eight genes before and after interaction with epithelial cells.
Additionally to the presence of the egfp reporter gene by PCR analysis, expression of the gene in the fungal transformants was verified by fluorescence microscopy analysis.
Semi-quantitative Reverse transcriptase-polymerase chain reaction (SqRT-PCR) analysis: Expression of the integrated gene was tested on the RNA extracts of the T1 plants that showed positive results after being sprayed with the Basta herbicide.
In order to derive the analysis expression of the probability distribution and noise, we transform the chemical master equation into a confluence hypergeometric equation [ 51, 53], using the probability-generating function.
In accordance with our proteome analysis, expression of the mitochondrial chaperone HSP60 was significantly decreased in yMSCs (0.6-fold P=0.009) and aMSCs (0.8-fold P=0.010) grown in aSerum.
In a multivariate analysis, expression of the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) was significantly related to worse locoregional control (LRC; HR: 2.2, 95% CI: 1.1 4.6; P=0.03), independent of lymph node metastases (HR: 5.6, 95% CI: 1.2 27.4; P=0.03) and extranodal spread (HR: 2.7; 95% CI: 1.2 6.5; P=0.02).
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However, the analysis expression of such (p^{ast}) in Theorem 3 may not be the optimal result.
In the tumor analysis, expression of Noxa was seen in all biopsies.
For analysis, expression levels of the housekeeping gene rp49 were used as an RNA loading control.
Fajas, L. et al. Organization, promoter analysis and expression of the human PPARγ gene.
Northern blot analysis demonstrated expression of the variant mRNAs.
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