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To assess clonality, we used an RT-PCR-based TCR Vβ-repertoire analysis detecting 21 Vβ-families [17].
Fluorescence in situ hybridization (FISH) analysis detecting the rat and mouse genomic DNA and immunoelectron microscopy using anti-GFP revealed donor-derived cells.
Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains.
The single cell genome sequence reads were analyzed using BLASTX (e-10, 10 best hits) and lowest common ancestor algorithm (LCA) assignments using MEGAN [29], as well as GC content analysis, detecting high levels of contaminating reads (Figure S2).
The specificity of the Pax2 antibody was assessed by Western blot analysis, detecting 2 bands at 51 and 44 kDa, and by comparison of patterns of immunofluorescence to those seen with in situ hybridization [14].
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This analysis detected a prominent seed component with a molecular weight of 176 — the molecular weight of ODAP is 176.13 — which appeared to corroborate the earlier HPLC results.
This analysis detected 4-methyl steranes chemicals often made by tiny algae known as dinoflagellates.
GC MS analysis detected 27 bioactive compounds (Table 2).
Analysis detected all known variants in nuclear genes.
Northern blot analysis detected a 2.1 kb message in trophozoites.
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CEO of Professional Science Editing for Scientists @ prosciediting.com