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Steady-state culture conditions were deemed to have been established once biomass and dissolved oxygen measurements had remained constant over three residence times (three vessel volume changes), at which point culture samples were taken for analysis, designated as non-induced steady-state sorbitol (SS) samples.
We isolated a stable transgenic line for analysis designated Tg(sox10 DNhsaerbb4-rfp), from now on called Tg(sox10 DNhsaerbb4-rfp
Our analysis designated the energy/bit metric as the most appropriate metric for energy-constrained low-resource designs.
Three genes, bHLH (UniProt ID: D7T931), AG1 (UniProt ID: Q40168) and ZF (UniProt ID: B9H0X4), were identified as being overexpressed in ripe fruit in RNA-seq data analysis and thus were designated for further confirmation.
The vacuum-tube manufacturing and analysis facility in Franklin Hall was designated for transfer to the fourth-floor north wing of Phillips Hall.
Only differences of two-fold or greater that recurred in both replicates were designated for further analysis.
Specimens designated for transcriptomics analysis and those serving as backup were snap-frozen in liquid nitrogen and stored at −80 °C.
Samples designated for histology analysis were placed in paraformaldehyde 4% solution and later treated as described in the next section.
The cellular suspension designated for immunocytochemical analysis was prepared by cytospin and fixed with acetone and methanol.
Each sample designated for FCM analysis was divided into two parts for detecting viability (apoptosis and necrosis) and for detecting CD14 and CD11b expression.
Cells designated for phototoxicity analysis were irradiated for 10 min as indicated above with white light at an irradiance of 16 J/cm/min.
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CEO of Professional Science Editing for Scientists @ prosciediting.com