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For molecular analysis, cultivated bacteria were resuspended in saline and inactivated at 98°C for 20 min. Total DNA was extracted by using the DNA-purification Kit (QIAGEN, Hilden, Germany).
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The goal should be tools and practices that enable both exploratory and repeatable data analysis, cultivate collaborative development and produce open easily exchanged data.
Plants for apoplastic barrier analysis were cultivated for 6 weeks.
Primary cultures of breast epithelial cells available for 2-DE analysis were cultivated from biopsy specimens of 23 breast cancer patients.
The metabolic flux analysis of Synechocystis cultivated under heterotrophic and mixotrophic conditions has been made based on 13C-metabolic flux analysis (13C-MFA) [72,73], and the metabolic regulation analysis has also been made with integration of different levels of information [74].
People read Benjamin not only (or even mainly) to learn about the particular topics he took on -- Belle Epoque fashions, Art Nouveau interiors, iron construction -- but also to absorb his methods of urban analysis and extravagantly cultivated sensibility.
During the analysis period (1964 2014), cultivated and rural settlement land constituted the largest coverage in the study area.
A comparative metabolite analysis of the cultivated, moderately freezing-sensitive T. aestivum cv.
For hMSC adhesion and proliferation analysis, cells were cultivated on collagen I, fibronectin, laminin I, poly--lysine (PLL) or 5% milk/PBS (blotto).
A genome wide transcriptome analysis of cells cultivated under both conditions [ 6] did not provide indications for significant differences in the expression of glycolytic isoenzymes.
In accordance with the transcriptomic analysis, E. anophelis cultivated in the presence of sublethal H2O2 concentration (20 mM) produced a significantly larger quantity of siderophore compared with E. anophelis cultivated in medium alone (fig. 4 B).
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