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Two independent researchers scored each sleep segment using Neuroscore's analysis console.
Statistical analysis relative to mRNA expression data was performed using Affymetrix® Transcriptome Analysis Console (TAC) Software.
Data analysis, statistical analysis, and generation of gene expression heat maps were performed using Affymetrix® Transcriptome Analysis Console (TAC) Software.
The eight CHP files were then imported into the Affymetrix® Transcriptome Analysis Console (TAC) 2.0 (build 2.0.0.9), using the "Gene Level Differential Analysis" option.
Every job is submitted to a high-performance computing cluster and the user can follow its progress via an analysis console.
Raw data quality control and normalization were performed by using the Affymetrix Expression Console, whereas differentially expressed genes were determined with the Affymetrix Transcriptome Analysis Console (one-way analysis of variance P value of less than 0.05, fold change of more than or less than 2, false discovery rate of less than 0.05).
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Microarray data (CEL files) were generated using default Affymetrix microarray analysis parameters (Command Console suite software, Affymetrix).
As we did not have automated, immediately available, on-line volumetric analysis at the CT console during the recruitment time period, the following perfusion CT criteria were based on visual assessment using vendor software perfusion maps.
The local police was aided by the Maine State Police Computer Crimes Unit, which conducted a forensic analysis of the PlayStation consoles and cell phones to ultimately obtain an arrest warrant.
Data analysis was performed with Genotyping Console (Affymetrix).
The analysis was performed using Genotyping Console 3.0.2 (Affymetrix) software.
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