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qRT PCR and western blot analysis confirmed a decrease in TOPK at the mRNA (P<0.05) and protein levels which was more intense in the early stage.
Indeed, qRT-PCR analysis confirmed a decrease of the alpha-globin mRNA/beta-globin mRNA ratios, in agreement with the known molecular alterations of the disease.
The 3-dimensional video analysis confirmed a decrease in cytosolic H-Ras and an increase in membrane localization of H-Ras to the plasma membrane and Golgi (Supplementary Movie 5).
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Statistical analysis confirmed a significant decrease in BrdU+cells in the SVZ of irradiated animals compared with control animals at all points examined (ANOVA, p<0.05, Newman-Keuls).
Western blot analysis confirmed a sharp decrease in both cyclin D1, Cdk 4 and Cdk 6 levels, apart from other cell cycle proteins like cyclin B1 and p21, after 72 h of hUCBSC treatment, when compared with control cells, U251 and 5310 (Fig. 2A).
Blinded dermatopathological analysis confirmed a significant decrease in skin thickness and improvement in skin morphology.
Quantitative real-time PCR and western blot analysis confirmed a significant decrease in c-FLIP expression following transfection with shRNA.
Immunohistological analysis confirmed a similar decrease in microvessel density and increase in size of vessels with a diameter larger than 30 μm.
Real-time PCR analysis confirmed a significant decrease in the level of csf-1r mRNA in all cell lines treated with csf-1r -specific siRNA, whereas the expression of this receptor was significantly increased in all the cell lines treated with CSF-1.
qRT-PCR analysis confirmed an ∼80% decrease in FXN mRNA in all FRDA iPSC clones, as compared with control iPSCs (Fig. 1C).
Survival analysis confirmed an association between decreased HES1 immunosignal and shorter DFI.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com