Sentence examples for analysis by real time from inspiring English sources
To validate differential expression results a subset of the genes identified in the subtraction libraries were selected for analysis by real time PCR (RTPCR).
Analysis by real time RT PCR (Fig. 5) confirmed that Sall3 is expressed at high level in mouse embryo until E15.5.
To confirm the differential regulation pattern of genes observed by microarray analysis after CS/CPB, we selected 13 genes for confirmation analysis by real time quantitative RT-PCR (qPCR).
Specific primer pairs for each gene (Table S4) were used for expression analysis by real time PCR performed with the SYBR Green PCR Master Mix kit (Applied Biosystems, Foster City, CA, USA) using the 7300 Real-Time PCR System (Applied Biosystems).
Although we have observed that PML Ib can be induced by IFNs, our quantitative analysis by real time PCR indicated that IFN-γ increased all PML isoforms, while HSV-1 infection favored an increased ratio of cPMLΔ5&6 to total PML.
DNA samples were recovered and subjected to analysis by real time PCR amplification.
Quantitative analysis by real-time PCR of mRNA levels in different tissues revealed a predominance of the transcripts for all three genes in the midgut, while analysis during development revealed greatest abundance in mRNA during the 3rd-instar.
cDNA was diluted 10× prior to gene-specific analysis by real-time RT-PCR using an iCycler MyiQTM system (Biorad Laboratories, Hercules, USA).
Quantitative analysis by real-time PCR confirmed the increase in GDNF mRNA induced by LY379268 at 3 h and showed a residual effect at 6 h that was not detected by in situ hybridisation analysis (Fig. 2A).
To investigate correlations between ALK and PHOX2B expression, we first carried out transcription analysis by Real-time RT-qPCR in a panel of 13 NB cell lines and additional control samples (see below).
We have now developed such a technique for the harvesting by LMD of fluorescent cells from brain tissue with subsequent RNA isolation and gene expression analysis by real-time PCR and microarrays.
