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After controlling for diagnosis, age, sex, BMI, analysis batch, and self-reported childhood trauma we found that women had significantly lower KynA/3HK and KynA/QA ratios than men, and that these results were driven by a decrease in KynA.
Analytic accuracy and precision were tested by concurrent analysis of quality control materials in the same analysis batch as the unknown specimen.
Two quality control samples were run per analysis batch.
To allow large-scale analysis, batch search can be implemented for the search by target gene.
Each analysis batch contained 16 unknowns, 2 method blanks, and 2 quality control specimens.
This sample series was included as part of a standard analysis batch of 24 samples.
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All analysis batches contained a sequence of a hexane blank, QC Aroclor check standard, a congener check standard, the method blank, six unknown samples, and either a method control sample or unknown sample duplicate.
The use of ICCs to estimate the agreement between analysis batches differs from their use as an index of temporal reliability.
The standard deviation for quality control samples in all analysis batches was less than 15%, and the results of method validation were specific, precise, and repetitive.
The intraclass correlation coefficient ICCs for absolute agreement between the two analysis batches ranged from ICC (2,1) = 0.47 to ICC (2,1) = 0.96 (for E1 and SHBG respectively, Table 4).
The methods were evaluated through a combination of SEM analysis and batch dissolution experiments.
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