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Square planar complexes were obtained as only products with good yield and were fully characterised by EI MS, NMR (1H, 13C) spectroscopy, elemental analysis, and, in the case of three complexes, by X-ray investigation.
All compounds were characterized by 1H, 13C, 15N, 119Sn NMR, mass spectrometry, IR, elemental analysis and in the case of compounds 4a, 4b, 4d and 4e by X-ray diffraction analysis.
Their structures were clearly verified by IR, 1H NMR, EI-MS spectroscopy and elemental analysis, and in the case of compound 5a, analyzed by single-crystal X-ray diffraction further.
These samples were extensively characterized with respect to BRCA1 and BRCA2 status with mutational and mRNA expression analysis and, in the case of BRCA1, promoter methylation and protein expression levels were also analyzed.
Confirmed sequences were subjected to further bioinformatic analysis and, in the case of dystrotelin, to preliminary functional and expression analysis.
All patients provided written informed consent prior to DNA analysis and in the case of children, the consent was given by the parent/legal guardian.
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And in the case of North Korea, as in Iraq, the immediacy of the threat depends on whose analysis seems most compelling.
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In the case of ITT analysis, we applied the LOCF (Last Observation Carried Forward Analysis) rule.
In the case of meta-analysis, the meta means to combine the results of several related studies or experiments.
Therefore in the case of TFs the analysis was simpler.
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