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Cells were treated with TNF-α and the supernatant, used for analysing, was collected 48 h after the start of the experiment.
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An additional sample analysed was collected in Antula district in 2007 (A-2007) (Figure 1).
Blood for chemistry, hematology and PBMC for mRNA analyses was collected a week later, and rabbits euthanized 7 or 8 days after the final i.v. boost.
Blood for serum analyses was collected by cardiac puncture.
Data used for the present cross-sectional analyses was collected from May 2010 to September 2013.
The blood for complement analyses was collected in EDTA tubes (Becton Dickinson, Plymouth, UK) and kept on ice until centrifugation.
Blood for insulin and C-peptide analyses was collected in dry tubes for coagulation (20 min at room temperature), and into lithium heparin tubes for paracetamol measurement.
Blood for haematological analyses was collected in 4 mL tubes, with EDTA (ethylenediaminetetraacetic acid) as anticoagulant (Vacuette®, Greiner Bio-One International AG, Kremsmünster, Austria).
Data used in the analyses was collected once at the beginning of the academic year, and again at the end of the academic year.
The paint samples analysed were collected from: Huaca de La Luna, Peru.
Blood samples for pharmacokinetic analyses were collected, and safety and tolerability were assessed.
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