Exact(2)
After 30 sec, 600 µl acridine orange (AO; Sigma) diluted in AO staining solution (37 mM citric acid, 126 mM Na2HPO4, 1 mM EDTA, 0.15 M NaCl, pH 7.4 with AO added fresh to a final concentration of 6 µg/ml) was added to the sperm, mixed and incubated for 3 min at room temperature before analysing using a fluorescent assisted cell sorting (FACS) machine.
Cells were subsequently incubated in the dark (22°C, 30 mins) before analysing using a Tali Image-Based Cytometer (Invitrogen, USA).
Similar(58)
Data were analysed using a SI8000C analyser (Sony).
Blood count was analysed using a Sysmex analyser (XE 2100).
The editor gave us permission to conduct a new set of analyses using a different operational definition of consensus".
Micrographs were analysed using a custom made algorithm.
Data was analysed using a custom Matlab (Mathworks) script.
Attacks can be analysed using a mixture of digital forensics and traditional investigative methods.
Variables were analysed using a mixed model.
saplings were analysed using a multivariate method.
Samples were analysed using a Leica TCS SP8 confocal microscope using a 40x original magnification.
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