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Therefore, to elucidate the cellular role of sacsin we have adopted a strategy of analysing the function of specific domains and siRNA.
To fully exploit this vast amount of information it is essential that efforts are made to improve and extend the range of tools available for analysing the function of genes in vivo.
Human and zebrafish gene function is often interchangeable, and introducing a human gene (by mRNA injection or as a transgenic line) to replace the zebrafish version to 'humanise' the fish is valuable for analysing the function of human gene variants (Singh et al., 2011).
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These findings open new perspectives on elucidating the mechanism of ABA response, and provide clues for analysing the functions of chloroplast proteins in regulating root growth.
However, the most effective way of analysing the functions of essential genes is through temperature sensitive (ts) mutants and, to date, such mutants have been isolated in only one gene, nsfA, a gene whose function is required for many steps in intracellular membrane transport.
Our study reinforces the importance of analysing the functions of a transcription factor in different cellular and signalling environments.
Despite the differences in the quantity of differential gene expression, a wide overlap was detected when analysing the functions of regulated genes.
We decide to investigate further by analysing the functions of the co-localised complexes: "DNA synthesome complex" and "TNF-alpha/NF-kappa B signaling complex 5".
To date, the studies analysing the functions of Egr-1 have been contradictory, with reports of both cytoprotective and pro-apoptotic functions in tumour cells (Huang et al, 1998a; Virolle et al, 2001).
Recently, progress has been made in analysing the functions of stress-inducible genes, not only to understand the mechanisms of drought stress but also to improve the drought tolerance of plants by gene transfer.
We suggest using a technique, called 'sequence capture', to sequence a large number of genes that could be used for analysing the functions of environmental communities at a significantly higher resolution than what has been possible with other approaches.
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