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(a) Schematic of analysing the function of ILSCm LP pathway.
In addition, prior to the study described here, there has been no animal model for analysing the function and mechanisms of the EBERs in vivo.
Therefore, to elucidate the cellular role of sacsin we have adopted a strategy of analysing the function of specific domains and siRNA.
To fully exploit this vast amount of information it is essential that efforts are made to improve and extend the range of tools available for analysing the function of genes in vivo.
Human and zebrafish gene function is often interchangeable, and introducing a human gene (by mRNA injection or as a transgenic line) to replace the zebrafish version to 'humanise' the fish is valuable for analysing the function of human gene variants (Singh et al., 2011).
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These findings open new perspectives on elucidating the mechanism of ABA response, and provide clues for analysing the functions of chloroplast proteins in regulating root growth.
In addition, the efficient transduction and expression with enhanced adenoviral transfer of therapeutic genes into primary keratinocytes provides a powerful tool for analysing the functions and the regulation of a gene of interest in vitro.
Our study reinforces the importance of analysing the functions of a transcription factor in different cellular and signalling environments.
Despite the differences in the quantity of differential gene expression, a wide overlap was detected when analysing the functions of regulated genes.
We decide to investigate further by analysing the functions of the co-localised complexes: "DNA synthesome complex" and "TNF-alpha/NF-kappa B signaling complex 5".
To date, the studies analysing the functions of Egr-1 have been contradictory, with reports of both cytoprotective and pro-apoptotic functions in tumour cells (Huang et al, 1998a; Virolle et al, 2001).
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