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In the case of the latter, the DNA quality was demonstrated by analysing in duplicate four four-fold serial dilutions of each DNA extract (inhibition runs) using the validated soybean-specific reference system (lectin gene: http://gmo-crl.jrc.ec.europa.eu/summaries/40-3-2_validated_Method.pdf .jrc.ec.europa.eu/summaries/40-3-2_validated_Method.pdf .jrc.ec.europa.eu/summaries/40-3-2_validated_Method.pdf
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All samples were analysed in duplicate.
The samples were analysed in duplicate by each laboratory along with a solution of PCDD/F standards and reference sediment.
Five unifloral and eleven multifloral samples were collected and analysed in duplicate (see Table 4 for description).
Samples were analysed in duplicate (patient samples from BL and FU were run on the same plate).
Samples of feed ingredients, diets and fish fillets, liver and kidney were analysed in duplicate using standard methods (AOAC 1997).
In order to prevent this, blanks (HPLC water without analytes or an internal standard solution) were extracted and analysed in duplicate in every sequence (begin, middle and end) as control for carry over and background concentrations.
Each miRNA was analysed in duplicate.
All samples were analysed in duplicate in digested and nondigested conditions.
For every primer pair, four different cDNA concentrations were analysed in duplicate.
At least four independent samples of each region were analysed in duplicate TaqMan assays.
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