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The differential expression patterns in Cx and Ox observed in microarrays analyses were validated for all selected genes.
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The method used for analyses were validated over a concentration range 1.5 500 μg/mL for gastrodin in plasma, and a concentration range 0.05 1.5 μg/mL for gastrodigein in brain.
Laser diffraction analyses were validated against sieve-type measurements for the same material.
Correlation analyses were validated by the Spearman ρ correlation test for continuous non parametric variables and by the κ test for categorical variables.
Correlation analyses were validated by the Spearman rho correlation test for continuous nonparametric variables and by the kappa test for categorical variables (Landis and Koch, 1977).
The ChIP analyses were validated using single ChIP-qPCR with different antibodies for 11 42 randomly selected peaks (Supplementary Table S5).
DCE analyses were validated using Stata 14.1.
Methods for extraction of the drugs from hair were developed and subsequent ELISA analyses were validated in-house.
All analyses performed were validated for pig plasma.
The prespecified cutoff point for CD8+ iTIL (0 vs. ≥1) used for the analyses was validated in our previous studies [ 16, 35].
The theoretical analyses are validated by finite element analyses.
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