Protein levels visualized by immunoblot analyses were quantified using the NIH National Institutes of Healthh) ImageJ software (http://rsb.info.nih.gov/ij/) and normalized against the GAPDH loading control as described in Uittenbogaard et al. (2010).
One and two-dimensional 1H NMR analyses were performed and metabolites were quantified by the ERETIC method.
The K8 and Ki67 expression levels in treated and untreated NCI-H69 SCLC and GOT 1 midgut tumour xenograft sections were quantified by analysing DAB staining with the ImageJ programme developed by the National Institute of Health [40].
The effects of taxol and nocodazole on T cell migratory polarity were quantified by analysing F-actin and α-tubulin distribution.
Protein eluates were analysed by SDS-PAGE and were quantified by A280 measurement.
Nontechnical skills were quantified by analysing videotapes and scoring the Anaesthetist NonTechnical Skills [ 2].
Immunoblots were quantified by analysing bands using densitometry or Image J. Cell viability/proliferation assays were performed using MTT assays, which relies on the ability of live cells to convert the yellow tetrazolium salt (MTT (Sigma) to dark blue, water-insoluble MTT formazan crystals.
Protein expression levels on western blots were quantified by densitometry analyses using the ImageJ software.
To help understand metabolic shifts associated with elevated glycerol, protein expression levels were quantified by LC/MS/MS analyses.
Conditioned media were collected and Aβ42 and total Aβ levels were quantified by ELISA analyses.
