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Colonies of 20 potential RQ7/pDH10 transformants were picked for further analyses.
They were picked for their color.
were picked for maturity analysis.
The individual clones were picked for sequencing.
All the positive colonies were picked for screening analysis.
Resistant clones were picked for expansion and characterization.
Mated hermaphrodites were picked for egg lays on RNAi plates.
Only small proportions of the libraries were picked for sequencing.
Colonies lacking PCR products were picked for further verification.
Colonies with more intense fluorescence were picked for further investigation.
Colonies were picked for each sample and PCR amplified using T7/T3 primers, followed by Exonuclease 1 (New England Biolabs) digestion and direct Sanger sequencing (ABI 3730xl; Applied Biosystems) Analyses were performed using SPSS statistical software (IBM, Armonk, NY, USA).
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