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Based on the participants' responses to the VHI, statistical analyses were, initially, performed to assess validity and reliability, and then to evaluate group differences between the pathological and control groups and among the different pathological groups included in the study.
XRF analyses were initially performed in situ on the glass lamp using an ELIO portable X-ray fluorescence spectrometer (XGLab), equipped with a rhodium tube and 1 mm spot size.
Whole brain analyses were initially performed using both normalised data (co-registered to the SPM EPI template) and non-normalised data (co-registered to each participant´s T1 anatomical image).
Analyses were initially performed on separate data sets of the Rotterdam Study (prevalent AMD, incident AMD), the AMRO-NL case-control study (in parallel), and subsequently conducted on combined data from the Netherlands (of population-based and case-control groups) and data of all population studies combined.
Statistical analyses were initially performed by AR and CS.
All analyses were initially performed with sex of participants as a between-subjects' factor.
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Analyses of nerve conduction studies were initially performed with a Toennies electromyograph (Toennies, Würzburg) and later with a digital Neurosoft-Evidence 3102 electromyograph (Schreiber & Tholen Medizintechnik).
Separate 'crude' interaction analyses adjusted for each interaction term, group, and baseline value of the outcome measure were initially performed.
CT scan was initially performed and analyzed.
Bivariate logistic regression was initially performed.
All linear regression analyses were, however, initially performed with the advertisement-recruited participants' values placed alternately on the y- and x-axes.
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