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Survival analyses were estimated by the Kaplan-Meier method [ 3].
Survival analyses were estimated by the Kaplan-Meier method and were compared using the log-rank test.
Additive-genetic and residual variances, and, used as prior information in the statistical analyses, were estimated by ASReml [ 20] utilizing all phenotyped bulls in the pedigree.
For each dataset, genetic differentiation (F ST ) between the clades identified through TCS and phylogenetic analyses were estimated by also using DnaSP.
Precisions in the point estimates for all analyses were estimated by calculating 95% confidence intervals (CI), with the exception of the correlation coefficient where the p-value was used.
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The confidence of nodes in amino acid analyses was estimated by 1,000 bootstrap replicates generated using SEQBOOT and compiled in a consensus tree with CONSENSE.
The threshold for reporting association to MDD in the single marker analyses was estimated by a family-wise error rate (FWER), using Bonferroni's method.
The protein abundance identified by 1-DE and shotgun analyses was estimated by the exponentially modified Protein Abundance Index (emPAI) [ 58], which was automatically calculated by the MASCOT search engine.
The accuracy of the hydrocarbon analyses was estimated by recoveries from the spiked blank, which ranged from 69 to 111% for 17 different PAHs (naphthalene, 102%; fluorene, 85%; dibenzothiophene, not determined; phenanthrene, 98%; chrysene, 83%).
Morphometric analyses were estimated independently by two observers that had no prior knowledge of the patients' clinicopathologic data.
Correlations between miR-195 and miR-497, and miR-483-3p miR-483-3p miR-483-3pevels were andessed by PUMAson's correxpressionalevelsand P values were estimassessedpermuting the samples.
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