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The percentages of tumour purity in these tissues and adjacent sections used for RNA and protein analyses were established by routine histopathological analyses.

The statistical analyses were established using Analysis of Variance (ANOVA) followed by the Tukey-Kramer multiple comparison test [ 23].

Within-herd prevalence was established by individually analysing a maximum of 50 animals from a selection of 9 pool-positive herds (4 sheep, 1 beef and 4 dairy cattle) accounting for a total of 418 animals.

In parallel to the microarray analyses, quantitative PCR expression ratios were established by comparing normalised relative expression at each time point after clostridial challenge to that before the challenge (D0 PI) within each treatment group.

Open image in new window Fig. 2 1H–1H COSY and HMBC correlations for 1 and 5. Connectivity among the above mentioned fragments and the contribution of the three-ring system were established by analysing 1H, 13C long-range correlations extracted from HMBC experiments (Fig. 2).

Baseline probabilities and probabilities of transition from one state of health to another over time were established by analysing the management modalities of all consecutive new cases of lung cancer diagnosed between 1 July 1998 and 30 June 1999 in a representative French national sample of health-care centres managing lung cancer.

The structures of these compounds were established by spectroscopic analyses including APCI-HR-MS aNMRNMR.

Bioassay-guided fractionation of the active fractions yielded compounds α-amyrine (1), β-amyrine (2), ursolic acid (3), oleanolic acid (4), betulinic acid (5), brugierol (6), iso-brugierol (7), kaempferol (8), quercetin (9) and quercetrin (10), which were established by spectroscopic analyses including APCI-HR-MS and NMR (1H, 13C, DEPT 135, DEPT 90, COSY, NOESY, HMBC and HMQC).

Authenticity of these complexes were established by competition analyses using 100 and 200× (Figure 2B, lanes 2 and 1, respectively) molar excess of oligonucleotides corresponding to the putative AP-1 binding site spanning −647 to −657 of the hres gene promoter.

Orthology relationships for the other 44 sequences from the set were established by individual analyses.

Chromosome assignments for 861 SNPs were determined by twopoint analysis, and positions for 735 SNPs were established by multipoint analyses.