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The elemental analyses were done using a CHNS-932 (LECO) and the Inductively Couple Plasma Atomic Emission Spectrometry (ICP-AES), with a Perkin Elmer Spectrophotometer model Optima 2000DV under standard condition.
All analyses were done using a blind procedure.
All analyses were done using a Rheos 2200 HPLC instrument (Flux Instruments, Switzerland) with an LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific), equipped with an electrospray ionization probe.
Statistical analyses were done using a paired t test.
Moreover, statistical analyses were done using a linear mixed model.
Biometrical analyses were done using a siascope tool.
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The analyses were done using an argon-flow of 15 mL min−1 and using a collision reaction interface with H2 and He as skimmer gases for selected elements.
Mass spectrometric analyses were done using an API 4000™ quadrupole mass spectrometer (Applied Biosystems/MDS Sciex Toronto, Canada) equipped with an electrospray ionization (ESI) source.
All analyses were done using an intention-to-treat basis, which included all randomized participants.
Post hoc analyses were done using an unpaired Student's t test for continuous data and the Mann–Whitney U test for discrete data.
At the University Hospital Heidelberg Interdisciplinary Operative Intensive Care Unitt) point-of-care blood gas analyses are done using a RAPIDLab® 1265 blood gas analyser (BGA; Siemens Healthcare Diagnostics GmbH, Bad Nauheim, Germany).
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