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Samples for RNA analyses were collected by filtration during fermentation after consumption of 15 g/L sugars.

The data from the HPLC-MS/MS analyses were collected by using Analyst 1.4.1 software (AB Sciex, Toronto, Ontario, Canada).

In addition, risk factors for both analyses were collected by self-report, which may have been inaccurate.

Mucosal samples for gene expression and protein analyses were collected by scraping the next 4 cm of proximal and distal colon with new microscope slides.

Data used in these analyses were collected by a structured questionnaire that covered health; demographic, socioeconomic and psychosocial factors; health behaviours and nutrition.

At the same time, blood samples for metabolites and hormones analyses were collected by coccygeal venopunction into heparinized tubes from 94 cows, centrifuged at 3000 Xg for 20 min and plasma was stored frozen (-20°C) until further analysis.

Blood for serum analyses was collected by cardiac puncture.

Data for the cost-effectiveness and cost-utility analyses are collected by using a retrospective cost interview.

Toe clip samples for genetic analyses were collected under ethics approval granted by the Department of Conservation New Zealand Animal Ethics Committee (permit no. 181).

Hormone Analyses Blood samples were collected by cardiac puncture from mice anesthetized with isoflurane.

For malaria parasites analyses; blood samples were collected by the standard finger-prick method and thick and thin smears prepared on labelled slides.