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MS n analyses were acquired on the LCMS-IT-TOF mass spectrometer (Shimadzu, Kyoto, Japan).
Analyses were acquired as a time-resolved signal with 20 s of background followed by 40 s of counts on ablated material.
The powder X-ray diffraction (XRD) analyses were acquired using Bruker AXS D8 Advance X-ray diffractometer, Germany with Cu-Kα radiation source filtered (λ = 1.5406 Å) at 40 kV and 30 mA.
All the images for quantitative analyses were acquired under nonsaturating exposure conditions.
Images for all MSD analyses were acquired at 15 fps.
In all cases, images for these analyses were acquired at 4.2 fps.
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For ribosomal analyses, MS1 spectra were acquired at 240k resolving power (at m/ z 400) and product ion spectra after UVPD were acquired for the top three most abundant precursors by averaging three scans at 240k resolving power.
Images for all analyses in the figure were acquired at 15 fps.
Phylogenetic analyses suggest that genes were acquired repeatedly by divergent viruses or viral strains of the Nimaviridae.
For MS analyses, typically 800 spectra were acquired for each spot in the reflector positive mode in the mass range of 800 to 5000 m/z, with 15 ppm mass tolerance (external calibration).
Images for all analyses in the figure were acquired at a lower frame rate of 4.2 fps; this is because the small number of Cy3 dyes that could be incorporated into the short HLE RNA necessitated imaging with a higher exposure time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com