Integration of the data by Procrustes and Co-inertia analyses was achieved using the functions "procrustes" and "cia" of "Vegan" [ 44] and "Made4" [ 48] packages, respectively.
For all analyses, however, syntheses were achieved using a weighted linear combination of the study estimates so as to give more weight to studies of larger size.
Negative ion mode analyses of polar metabolites were achieved using a HILIC method under basic conditions.
Sensitivity as high as 3.9 × 10−2 A mol−1 L/cm2 and limit of detection as low as 8.4 × 10−9 mol L−1 were achieved using flow injection analyses.
Mass spectrometric analyses were performed using the following settings: nebulisation and desolvation were achieved using nitrogen gas at flow-rates of 50 and 900 L h-1, respectively.
Pairwise comparisons were achieved using Mann Whitney-U test.
For the data stored in the workset, a succession of analyses can be achieved using a number of implemented comparative genomics and molecular evolution analysis tools.
Genes were clustered based on expression profiles determined through the use of a post-hoc chi-square test statistic in pair-wise analyses and was achieved using custom Perl scripts, which are available upon request [ 26].
As only a limited number of cells can be irradiated per day, typically <25 000, most of the analyses on cellular signalling can only be achieved using semi-quantitative immunostaining methods in this study.
