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All analyses were achieved by a series of custom Perl scripts.
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Protein separation for western blot analyses was achieved by SDS-polyacrylamide gel electrophoresis.
Speed-up of the analyses is achieved by parallelizing certain steps and taking advantage of multithreading where possible.
Analyses were achieved in triplicate.
This is achieved by individually analysing comprising self-strengthened corrugated plates using the effective width concept whilst the effect of corner enhancement is taken into calculation.
With a sample volume of 2 mL, the optimal conditions in terms of total response values and reproducibility were achieved by performing analyses with a divinylbenzene/polydimethylsiloxane (DVB/PDMS) fibre, in an acidic pH (pH 2) with the addition of 0.59 g of NaCl, allowing the sample to equilibrate for 9 min and extracting at 68 °C for 24 min.
All statistical analyses except analysis of covariance (ANCOVA) were achieved by using SPSS v.13.
Specific analyses within the knocked down cells were achieved by gating with Venus fluorescence.
Analyses shown in Fig. 5A and B were achieved by scanning promoter ratios with bins of 51 (thin line) and 201 (thick line) promoters for average after sorting them according to their responses to ABA or wounding.
All of these were achieved by 2006.
Efficient reconstitution of membrane proteins for functional analyses can be achieved by dilution of a ternary mixture containing proteins, lipids and detergents.
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