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Using this approach in combination with complementation analyses, we validated the phenotypes of two novel mutants, MNN11 and HSL7, both of which are required for controlling peroxisome size (Figure 4).
44 To confirm the genes identified through the pathway analyses, we validated the result of the microarray analyses using qRT-PCR (figure 3).
Finally, based on transcriptional and morpho-physiological analyses, we validated the universal nature and functional conservation of selected shared DEGs in a fifth species, Brachypodium distachyon.
By conditional mosaic gain- and loss-of-function analyses, we validated the activity of those factors on regulating rx2 transcription and consequently modulating neuroretinal and RPE stem cell features.
Because these data were used in downstream analyses, we validated the data set by performing quantitative reverse transcription PCR (qRT-PCR) of twenty-seven geneselecteded from the RNA-Seq analysis (Additional file 2).
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We validated the WGS analyses by resequencing all MIRU loci via Sanger sequencing, which allowed us to assess the accuracy of using RD and PEM approaches to identify minisatellite variations.
Of the 9 miRNA sequences tested experimentally using Northern blot analyses, we successfully validated the presence of 8 of our predicted Ciona miRNA homologs in the RNA of the adult tissue of Ciona intestinalis.
These analyses further validated the role of SaSce9 for improving stress tolerance in plant by affecting different stress related pathways.
Comparisons with previously conducted experiments and detailed finite element analyses validated the accuracy of the model.
Analyses validated the relevance of a five-grade model for DM1 classification.
Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) analyses validated the formation of NA-COGH on the glassy carbon electrode.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com