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For Bayesian analyses we employed the GTR+I+G model of nucleotide evolution.
For these analyses we employed the wing-specific MS1096.Gal4, a homozygous viable insertion in the second intron of the Beadex gene on the X chromosome.
For the MSP analyses, we employed the most widely-used conservative thresholds, originally proposed by Lander and Kruglyak (1995) [48] (LK-significant = 2.2E-05, LK-suggestive = 7.0E-04 7.0E-04
To complement these analyses, we employed the Ba/F3 cell model system.
As in the BEAST analyses, we employed the WAG model for proteins and the GTR model for nucleotides.
For these analyses, we employed the item assessing overall satisfaction with the day before as the validation criterion.
Similar(48)
In the GEMS analyses we employ the latter approach.
For these analyses, we employed both the EGFR-expressing SCC61 cells, which were used for initial candidate compound screening (Fig. 3a), and a second highly EGFR-dependent cell line, A431 human squamous carcinoma cells (Fig. 3b).
Thus the iterative analyses we employed struck a balance between the depth of analysis and tractability.
To assess the precision and the accuracy of the proteomic data in our analyses, we employed external calibration standards using the All-in-1 Peptide Molecular Mass Standard (Ciphergen Biosystems, Inc ., which allowed us to achieve a mass accuracy of approximately 1 Da in 10,000.
For the current analyses we employed previously described criteria 11 12 for the following four domains: (1) being free from major chronic disease; (2) having no major impairment of cognitive function; (3) having no major limitation of physical functions and (4) and having good mental health.
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