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For Bayesian analyses we employed the GTR+I+G model of nucleotide evolution.
Thus the iterative analyses we employed struck a balance between the depth of analysis and tractability.
To validate these analyses, we employed a 10-fold cross validation method by using all 25 samples.
For these analyses we employed the wing-specific MS1096.Gal4, a homozygous viable insertion in the second intron of the Beadex gene on the X chromosome.
For the MSP analyses, we employed the most widely-used conservative thresholds, originally proposed by Lander and Kruglyak (1995) [48] (LK-significant = 2.2E-05, LK-suggestive = 7.0E-04 7.0E-04
For our single-molecule AFM analyses, we employed in vitro mutagenesis to substitute the cysteine residues either individually or pairwise for alanines.
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In our subsequent analyses, we employ a number of different econometric methods.
In these analyses we employ two methods for quantifying the geographic range of fossil marine genera.
In the GEMS analyses we employ the latter approach.
In order to remove variables in the logistic regression analyses, we employ stepwise backwards elimination with p > 0.05 as the level for removing effects, while keeping age and gender as important sociodemographic variables and the other outcome (general or mental health) in order to control for them.
This 'ORFeome' serves as a foundation for subsequent functional genomic analyses and we employed the Gateway® technology to transfer these open reading frames into the yeast two-hybrid plasmids pAD (prey) and pDB (bait).
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